Technical information for the Phytotitre natural extract libraries

Technical information for the Puretitre natural compound library

Recombinant protein technical data sheets

Safety First - Safety information for Phytotitre projects

Project guides and outlines (laboratory projects)

Antibiotic drug discovery project outline
Cancer biology drug discovery project outline
Enzyme inhibitor drug discovery project outline
Immunology drug discovery project outline

Project guides and outlines (data analysis "dry" projects)

Project guides for data analysis projects in Microbiology
Project guides for data analysis projects in Cancer Biology

Method sheets for laboratory projects

Method Sheet 01 - Defrosting, opening and re-sealing the Phytotitre or Puretitre kits
Method Sheet 02 - Preparing a bacterial culture for screening in 96-well format
Method Sheet 03 - Plating bacterial cell cultures for screening with the Phytotitre kit
Method Sheet 04 - Challenging bacterial cultures with the Phytotitre extracts
Method Sheet 05 - Measuring bacterial growth by plate-based absorbance assay
Method Sheet 06 - Plating mammalian cell-lines for screening with the Phytotitre kit
Method Sheet 07 - Challenging mammalian cell-lines with Phytotitre extracts
Method Sheet 08 - MTT assay for mammalian cell metabolic activity
Method Sheet 09 - Crystal violet assay for mammalian cell biomass
Method Sheet 10 - Resuspending solvent-free extracts for further experimentation
Method Sheet 11 - Preparation of dose curves from individual extracts
Method Sheet 12 - Plating macrophages for cytokine production assay
Method Sheet 13 - Challenging macrophages with Phytotitre extracts and LPS
Method Sheet 14 - Measuring TNF-α secretion using the L929 cell bioassay
Method Sheet 15 - Screening for inhibitors of the enzyme β-galactosidase
Method Sheet 16 - Using a microplate reader to measure absorbance
Method Sheet 17 - Data analysis and chart plotting (bacterial growth data)
Method Sheet 18 - Data analysis and chart plotting (mammalian cell viability data)
Method Sheet 19 - Data analysis and chart plotting (L929 cell bioassay data)
Method Sheet 20 - Data analysis and chart plotting (enzyme activity data)
Method Sheet 21 - Statistical analysis of screening data
Method Sheet 22 - Statistical analysis of dose curve data

Method sheets for data analysis projects

Method Sheet 50 - Introduction to the Phytotitre and Puretitre libraries
Method Sheet 51 - Basic data handling using Microsoft Excel
Method Sheet 52 - Background correction of absorbance values
Method Sheet 53 - Calculation of bioassay ‘Z-prime’ score
Method Sheet 54 - Calculation of mean and SD of repeat experiments
Method Sheet 55 - Mapping experimental data to compound maps
Method Sheet 56 - Drawing appropriate charts of experimental data
Method Sheet 57 - Adding error bars to charts
Method Sheet 58 - Identifying ‘hits’ from a compound screen
Method Sheet 59 - Basic correlation analysis
Method Sheet 60 - Basic Student’s T-test using Microsoft Excel
Method Sheet 61 - Exploring the structural features of Puretitre compounds
Method Sheet 62 - Calculation of IC50 by 4 paramater curve fitting using Excel

Datasets for data analysis projects in Microbiology

Screen of the Puretitre library for inhibitors of growth of Micrococcus luteus
Screen of the Puretitre library for inhibitors of growth of Escherichia coli DH5-alpha
Screen of the Phytotitre library for inhibitors of growth of Staphylococcus aureus
Screen of the Phytotitre library for inhibitors of growth of Escherichia coli DH5-alpha

Datasets for data analysis projects in Cancer Biology

Screen of the Phytotitre library for inhibitors of growth of the A2780 tumour cell-line
Screen of the Phytotitre library for inhibitors of growth of the HepG2 tumour cell-line
Screen of the Phytotitre library for inhibitors of growth of the MCF-7 tumour cell-line
Screen of the Phytotitre library for inhibitors of growth of the PC3 tumour cell-line

Notice for Students and Educators
Disclaimer: These resources are provided for educational purposes only. The user’s University Supervisor remains the Principal Investigator and the sole party responsible for the safe conduct, risk assessment, and ethical oversight of all laboratory work. Caithness Biotechnolgies Ltd. accepts no liability for any injury, loss, or damage resulting from the application of the advice or protocols provided herein.