Technical information for the Phytotitre natural extract libraries

Technical information for the Puretitre natural compound library

Recombinant protein technical data sheets

Safety First - Safety information for Phytotitre projects

Project guides and outlines (laboratory projects)

Antibiotic drug discovery project outline
Cancer biology drug discovery project outline
Enzyme inhibitor drug discovery project outline
Immunology drug discovery project outline

Project guides and outlines (data analysis "dry" projects)

Project guides for data analysis projects in Microbiology
Project guides for data analysis projects in Cancer Biology

Method sheets for laboratory projects

Method Sheet 01 - Defrosting, opening and re-sealing the Phytotitre or Puretitre kits
Method Sheet 02 - Preparing a bacterial culture for screening in 96-well format
Method Sheet 03 - Plating bacterial cell cultures for screening with the Phytotitre kit
Method Sheet 04 - Challenging bacterial cultures with the Phytotitre extracts
Method Sheet 05 - Measuring bacterial growth by plate-based absorbance assay
Method Sheet 06 - Plating mammalian cell-lines for screening with the Phytotitre kit
Method Sheet 07 - Challenging mammalian cell-lines with Phytotitre extracts
Method Sheet 08 - MTT assay for mammalian cell metabolic activity
Method Sheet 09 - Crystal violet assay for mammalian cell biomass
Method Sheet 10 - Resuspending solvent-free extracts for further experimentation
Method Sheet 11 - Preparation of dose curves from individual extracts
Method Sheet 12 - Plating macrophages for cytokine production assay
Method Sheet 13 - Challenging macrophages with Phytotitre extracts and LPS
Method Sheet 14 - Measuring TNF-α secretion using the L929 cell bioassay
Method Sheet 15 - Screening for inhibitors of the enzyme β-galactosidase
Method Sheet 16 - Using a microplate reader to measure absorbance
Method Sheet 17 - Data Management & Integrity and Good Laboratory Practice
Method Sheet 18 - Background correction and normalisation of bacterial growth data
Method Sheet 19 - Background correction and normalisation mammalian cell viability
Method Sheet 20 - Background correction and normalisation of enzyme activity data
Method Sheet 21 - Background correction and normalisation of L929 cell bioassay
Method Sheet 22 - Calculation of Z’ factor from screening assay data
Method Sheet 23 - How to define and identify hits from screening data
Method Sheet 24 - How to fit a 4PL curve and calculate IC50
Method Sheet 25 - How to perform a two way ANOVA to analyse dose curve data
Method Sheet 26 - Disk diffusion assays for inhibitors of bacterial growth
Method Sheet 27 - How to perform a one way ANOVA statistical analysis
Method Sheet 28 - How to prepare a non-polar herb extract
Method Sheet 29 - Measuring effects of hit extracts on bacterial growth rate
Method Sheet 30 - Statistical analysis of bacterial growth kinetics data
Method Sheet 31 - Measuring enhancement of sensitivity to existing antibiotics
Method Sheet 32 - Statistical analysis of antibiotic enhancement data
Method Sheet 33 - Advice for writing up - Microbiology projects
Method Sheet 34 - Advice for writing up - Cancer biology projects
Method Sheet 35 - Advice for writing up - Biochemistry projects
Method Sheet 36 - Advice for writing up - Immunology projects
Method Sheet 37 - How to insert a table of contents using Microsoft Word
Method Sheet 38 - How to perform a kinetic analysis of cell killing
Method Sheet 39 - Determination of enzyme inhibitor class
Method Sheet 40 - How to plot a Lineweaver-Burk chart
Method Sheet 41 - Dose response assays for macrophage TNF-α production
Method Sheet 42 - Compound interference test for the L929 cell bioassay
Method Sheet 43 - Measurement of TNF-α bioactivity by L929 cell bioassay
Method Sheet 44 - Mapping experimental data to extract or compound plate maps
Method Sheet 45 - How to perform a Student’s T-test using Microsoft Excel

Method sheets for data analysis projects

Method Sheet 100 - Introduction to the Phytotitre and Puretitre libraries
Method Sheet 101 - Basic data handling using Microsoft Excel
Method Sheet 102 - Background correction of absorbance values
Method Sheet 103 - Normalisation of measurements to a control condition
Method Sheet 104 - Calculation of bioassay ‘Z-prime’ score
Method Sheet 105 - Mapping experimental data to compound plate maps
Method Sheet 106 - Identifying ‘hits’ from an extract or compound screen
Method Sheet 107 - Plotting column charts to show mean and SD
Method Sheet 108 - How to perform a one way ANOVA statistical analysis
Method Sheet 109 - Basic correlation analysis using Microsoft Excel
Method Sheet 110 - Exploring the structural features of Puretitre compounds
Method Sheet 111 - Applying corrections for multiple testing
Method Sheet 112 - Calculation of IC50 by 4 paramater curve fitting using Excel
Method Sheet 113 - Advice for writing up - Antibiotic data analysis projects
Method Sheet 114 - Advice for writing up - Cancer biology data analysis projects

Datasets for data analysis projects in Microbiology

Screen of the Phytotitre library for inhibitors of growth of Staphylococcus aureus
Screen of the Phytotitre library for inhibitors of growth of Escherichia coli DH5-alpha
Screen of the Puretitre library for inhibitors of growth of Micrococcus luteus
Screen of the Puretitre library for inhibitors of growth of Escherichia coli DH5-alpha
Replication: Effects of dihydroartemisinin (DHA) on growth of Escherichia coli DH5-alpha

Datasets for data analysis projects in Cancer Biology

Screen of the Phytotitre library for inhibitors of growth of the A2780 tumour cell-line
Screen of the Phytotitre library for inhibitors of growth of the HepG2 tumour cell-line
Screen of the Phytotitre library for inhibitors of growth of the MCF-7 tumour cell-line
Screen of the Phytotitre library for inhibitors of growth of the PC3 tumour cell-line
Replication study of the top five hit extracts for inhibition of growth of the MCF-7 tumour cell-line
Dose response of extract #736 Schizandra for inhibition of growth of the MCF-7 tumour cell-line

Notice for Students and Educators
Disclaimer: These resources are provided for educational purposes only. The user’s University Supervisor remains the Principal Investigator and the sole party responsible for the safe conduct, risk assessment, and ethical oversight of all laboratory work. Caithness Biotechnologies Ltd. accepts no liability for any injury, loss, or damage resulting from the application of the advice or protocols provided herein.